CLINICAL AND MOLECULAR BIOCHEMISTRY

Educational goals:

-stimulate and enhance the new roles of pharmacists in the transition from dispenser to counselor and to prescriber of supplements and nutraceuticals;

- provide a theoretical knowledge of the main biochemical and biomolecular methodologies aimed at the research and measurement of physiological, pathological and above all predictive biomarkers;

- provide the theoretical knowledge of modern methodologies useful for scientific investigation, for the management of existing technologies and those deriving from scientific innovation in the field of biology applied to the study of cellular and animal systems in physio-pathological conditions

- stimulate the acquisition of the epistemological method that allows to reach critical and reflective skills on language, in the analysis of research projects, experimental protocols and results, organization of methods and scientific knowledge, as well as teamworking

Interactive Lectures with use of ppt slides containing movie clips, links to Web sites and lecture. Classroom activites will be carried out.

Should teaching be carried out in hybrid mode or remotely, it may be necessary to introduce changes with respect to previous statements, in agreement with the programme planned and outlined in the syllabus

"Information for students with disabilities and / or LSD

To guarantee equal opportunities and in compliance with the laws in force, students concerned can ask for a personal meeting in order to plan any compensatory tools and / or dispensatory measures, based on the didactic objectives and individual needs. For further information, it is also possible to contact the referent professor for  CInAP (Center for Active and Participatory Integration - Services for Disabilities and / or LSD) of our Department, Prof. Teresa Musumeci. "

GENERAL BIOCHEMISTRY,General chemistry, biology. Knowledge of cellular metabolic processes, cell biology, structure and function of proteins, lipids, nucleic acids, replication, transcription, translation. Knowledge of: splicing and alternative splicing mechanisms, restriction enzymes and their role, cloning vectors, enzymes and isoforms.

The new role of the Pharmacist and the Pharmacy of services in Italy.
The analytical cycle. Biological samples and their use in the laboratory: pre-analytical, analytical and reporting variability.
Self-analysis and enzymatic activities in Pharmacy.
Organ disease, malnutrition diseases (maldigestion, malabsorption, obesity), metabolic diseases (diabetes, dyslipidemia, metabolic syndrome and cardiometabolic disease) and related biomarkers.
Oxidative stress and related pathologies, self-analysis.
Nutrigenomics, nutrigenetics and epigenetics in disease's risk
Microbiota and Dysbiosis. Analysis of the microbiome and markers of impaired intestinal permeability.
Inflammatory pathologies. Vitamin D and its metabolome in different pathologies. Vitamin B6 and B12. Methylation and transulfuration cycle: physiological and pathological roles
Next generation sequencing: NGS; study of the whole exome and clinical exome: clinical applications. Proteomics
DNA damage and repair: Comet assay. Widely used techniques in clinical biochemistry, biomedicine, forensics and occupational medicine, diagnostics: immunochemical and electrophoretic techniques, PCR, RFLP. cDNA and notes on vectors. Recombinant DNA and applications.Omics Technologies

•Ciaccio-Lippi, Biochimica clinica e medicina di laboratorio. EDISES Eds 2017

•Janson-Tischler, Biochimica Clinica-  (Ed Italiana di m. Plebani)- Ediz. Piccin 2015

•Antonozzi, Gulletta, Medicina di Laboratorio Logica e patologia Clinica Piccin

•Michael Laposata. MEDICINA DI LABORATORIO NEL LABORATORIO CLINICO. Ediz. Piccin, 2012 (italian version); III edition in English

•R.R. Holmes, J,Weyers, A.,Jones: METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI,Ed. Zanichelli 2008;
Damiano Galimberti et al. Nutrigenomica ed epigenetica. Dalla Biologia alla clinica. EdRa 2017
Dai J Dale, et al Geni ai Genomi . Edises 2013
http://www.youtube.com/watch?v=GkdRdik73kU&feature=rel

The final exam will be oral and based on lectures, slides and textbooks

At the end of the course there will be an open-ended written test consisting of 30 multiple-choice questions and 5 open-ended questions. The test will receive a grade which will be taken  into account in the final exam.
Verification of learning can also be an online assessment, if conditions require it.

The final exam will be oral and based on lectures, slides and textbooks

At the end of the course there will be an open-ended written test consisting of 30 multiple-choice questions and 5 open-ended questions. The test will receive a grade which will be taken  into account in the final exam.
Verification of learning can also be an online assessment, if conditions require it.

Examples of questions:
- Applications and Potential of novel  Pharmacy of Services
- Different Biological Samples to Assess Biomarkers.
-Cardiometabolic Pathology and Biomarkers
- Application of Omics Sciences