LABORATORIO DI BIOTECNOLOGIE FARMACEUTICHE - LABORATORIO DI PREPARAZIONE ESTRATTIVA E SINTETICA DEI FARMACI A - L

Learning Objectives

• LABORATORIO DI BIOTECNOLOGIE FARMACEUTICHE

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  To provide the basic Biotechnological and Pharmaceutical knowhow used in Medicinal Chemistry

• LABORATORIO DI PREPARAZIONE ESTRATTIVA E SINTETICA DEI FARMACI

  Provide the concepts and general strategies of retrosynthetic analysis for the resolution of complex synthetic problems through rational analysis; provide a basic experience in purification methods, identification and characterization of reaction and extraction products.

  Acquired skills: theoretical and practical knowledge concerning the synthesis of organic compounds endowed with pharmacological activity. Theoretical and practical knowledge on purification methods and identification of the synthesized compounds.

Course Structure

• LABORATORIO DI BIOTECNOLOGIE FARMACEUTICHE

  The course includes 28 hours of classroom lectures and 30 hours of practical exercises in the laboratory.

  Classroom attendance is mandatory. The maximum limit of absences allowed is 30% of the total hours of lessons.

• LABORATORIO DI PREPARAZIONE ESTRATTIVA E SINTETICA DEI FARMACI
  • Frontal lessons 28 hours
  • Laboratory practice 30 hours

Detailed Course Content

• LABORATORIO DI BIOTECNOLOGIE FARMACEUTICHE

  biotechnological and biomolecular methods for drug design and production. Manipulation of genic expression and production of recombinant protein in prokaryotic and eukaryotic cells. Downstream processing of protein products. Therapeutic proteins. Citokines. Vaccines. Monoclonal antibodies. Oligonucleotides (ON): ON-medicinal Chemistry, pharmacokinetics and toxicology. Antisense technology, aptamers, decoy, anti-genes, ribozymes, RNA-interference, micro-RNA. Bioinformatics.

• LABORATORIO DI PREPARAZIONE ESTRATTIVA E SINTETICA DEI FARMACI
  • Drugs of extractive, synthetic and semi-synthetic origin.
  • Design of a synthetic method: means and development of bibliographic research.
  • Retrosynthetic analysis.
  • Detailed analysis of the syntheses and reaction mechanisms involved in the preparation of compounds for therapeutic use and in the synthesis of molecules of biological origin: strategies and methods.
  • Common equipments, instrumentations and laboratory methods.
  • Translation of a synthetic plan into practical applications.
  • Synthesis of some simple organic compounds.
  • Synthesis of organic compounds with potential or known pharmacological activity.
  • Application of different chromatographic methods and analytical and spectroscopic techniques for purification and identification of synthesized compounds

Textbook Information

• LABORATORIO DI BIOTECNOLOGIE FARMACEUTICHE
  1. Bibliography and notes provided in the course: http://studium.unict.it/dokeos/2019/
  2. D. J. A. Crommelin & R. D. Sindelar, BIOTECNOLOGIE FARMACEUTICHE, Zanichelli.
  3. B. R. Glick, J. J. Pasternak, BIOTECNOLOGIA MOLECOLARE, Zanichelli.
  4. M. L. Calabrò, Compendio di BIOTECNOLOGIE FARMACEUTICHE, EdiSES.
  5. A. Maggi, BIOTECNOLOGIE FARMACOLOGICHE, MASSON.
  6. E. Klegerman, M. J. Groves - Pharmaceutical Biotechnology - Interpharm Press, Inc.
  7. J. M. Pezzuto, M. E. Johnson - Biotechnology and Pharmacy - Chapman & Hall, New York-London.
  8. G. Walsh, D. Headon - Protein Biotechnology - J. Wiley & Sons.
  9. K. Faber -BIOTRANSFORMATIONS IN ORGANIC CHEMISTRY.- Springer,
  10. B. R. Glick, J. J. Pasternak - MOLECULAR BIOTECHNOLOGY, PRINCIPLES AND APPLICATIONS- Zanichelli .
  11. L. Alberghina, E. Cernia - Biotecnologie e Bioindustria - Utet.
  12. C. Quaglierini, M. Vannini - Chimica delle fermentazioni e laboratorio - Zanichelli
• LABORATORIO DI PREPARAZIONE ESTRATTIVA E SINTETICA DEI FARMACI
  • Stuart Warren, Paul Wyatt - Organic synthesis -The Disconnection Approach, 2nd Edition - J. Wiley & Sons (2009).
  • Stuart Warren, Paul Wyatt - Workbook for Organic Synthesis The Disconnection Approach, 2nd Edition - J. Wiley & Sons (2009).
  • John Leonard, Barry Lygo, Garry Procter - Advanced practical organic chemistry - CRC Press (2013).
  • Douglas S. Johnson, Jie Jack Li - The art of drug synthesis - Wiley (2007).
  • Ruben Vardanyan, Victor Hruby - Synthesis of Best-Seller Drugs - Elsevier (2016).
  • Andrew Streitwieser, Heathcock Clayton - Introduzione alla chimica organica - PICCIN (1979).
  • B.S. FURNISS, A.J. HANNAFORD, P.W.G. SMITH, A.R. TATCHEL - Vogel’s Textbook of Practical Organic Chemistry - Pergamon Press (1990).