Academic Year 2019/2020 - 3° Year
Teaching Staff: Barbara Maria Rita TOMASELLO
Credit Value: 7
Scientific field: BIO/12 - Clinical biochemistry and molecular biology
Taught classes: 35 hours
Exercise: 24 hours
Term / Semester:

Learning Objectives

Educational goals:

-stimulate and enhance the new roles of pharmacists in the transition from dispenser to counselor and to prescriber of supplements and nutraceuticals;

- provide a theoretical knowledge of the main biochemical and biomolecular methodologies aimed at the research and measurement of physiological, pathological and above all predictive biomarkers;

- provide the theoretical knowledge of modern methodologies useful for scientific investigation, for the management of existing technologies and those deriving from scientific innovation in the field of biology applied to the study of cellular and animal systems in physio-pathological conditions

- stimulate the acquisition of the epistemological method that allows to reach critical and reflective skills on language, in the analysis of research projects, experimental protocols and results, organization of methods and scientific knowledge, as well as teamworking

Course Structure

Interactive Lectures, with use of slides, some movies and lecture

Detailed Course Content

The new roles of the Pharmacist ; Self-analysis, biological samples: pre-analytical, analytical and reporting variability. Spectrophotometric quantification of enzymatic activities in Pharmacy.

Maldigestion and malabsorption disease. Cardiometabolic disease, diabetes, obesity and related biomarkers.

Oxidative stress: role and self-analysis in pharmacy

Traditional food and novel food, nutraceuticals and supplements

Epigenetics: its roles and its modulation.

Importance of oral, dermal and intestinal microbiota in humans. Analysis of the microbiome and biomarkers of altered intestinal permeability. Interrelations between epigenetics and microbiota.

Laboratory support in autoimmune and inflammatory diseases: role of vit.D and its metabolome: role of its related biomarkers in different pathologies. Vitamin B6 and B12.

Methylation cycle: physiological and pathological roles

New generation sequencing: NGS; study of the whole exome and clinical exome, analysis and use of raw data DNA modification enzymes. cDNA and outline of the vectors. Damage and DNA repair.

Fundamental principles and applications of gene cloning and DNA analysis.

Molecular Techniques widely used in clinical biochemistry, biomedicine, forensics and in occupational medicine: immunochemical and electrophoretic techniques, PCR, RFLP, RAPD. Comet assay.

Transgenic plants, biotechnological and biosimilar drugs, cloning from adult cells, edible vaccines

Textbook Information

•Ciaccio-Lippi, Biochimica clinica e medicina di laboratorio. EDISES Eds 2017

•Janson-Tischler, Biochimica Clinica- (Ed Italiana di m. Plebani)- Ediz. Piccin 2015

•Antonozzi, Gulletta, Medicina di Laboratorio Logica e patologia Clinica Piccin

•R. Forlani, Analisi di Laboratorio per operatori della salute, con CD ROM- ENEA Eds 2011


•R.R. Holmes, J,Weyers, A.,Jones: METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI,Ed. Zanichelli 2008;
Damiano Galimberti et al. Nutrigenomica ed epigenetica. Dalla Biologia alla clinica. EdRa 2017
Dai J Dale, et al Geni ai Genomi . Edises 2013