Academic Year 2020/2021 - 3° Year
Teaching Staff: Barbara Maria Rita TOMASELLO
Credit Value: 7
Scientific field: BIO/12 - Clinical biochemistry and molecular biology
Taught classes: 35 hours
Exercise: 24 hours
Term / Semester:

Learning Objectives

Educational goals:

-stimulate and enhance the new roles of pharmacists in the transition from dispenser to counselor and to prescriber of supplements and nutraceuticals;

- provide a theoretical knowledge of the main biochemical and biomolecular methodologies aimed at the research and measurement of physiological, pathological and above all predictive biomarkers;

- provide the theoretical knowledge of modern methodologies useful for scientific investigation, for the management of existing technologies and those deriving from scientific innovation in the field of biology applied to the study of cellular and animal systems in physio-pathological conditions

- stimulate the acquisition of the epistemological method that allows to reach critical and reflective skills on language, in the analysis of research projects, experimental protocols and results, organization of methods and scientific knowledge, as well as teamworking

Course Structure

Interactive Lectures with use of ppt slides containing movie clips, links to Web sites and lecture. Classroom activites will be carried out.

Should teaching be carried out in hybrid mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus

The final exam will be oral

At the end of the course, a free written test will be carried out, consisting of 30 multiple choice quizzes and 5 open questions. The test will receive an assessment which will be taken into account for the final exam.

Learning assesment may also be carried out electronically, should the conditions require it.

Detailed Course Content

The new role of the Pharmacist and the Pharmacy of services in Italy.
The analytical cycle. Biological samples and their use in the laboratory: pre-analytical, analytical and reporting variability.
Self-analysis and enzymatic activities in Pharmacy.
Organ disease, malnutrition diseases (maldigestion, malabsorption, obesity), metabolic diseases (diabetes, dyslipidemia, metabolic syndrome and cardiometabolic disease) and related biomarkers.
Oxidative stress and related pathologies, self-analysis.
Nutrigenomics, nutrigenetics and epigenetics in disease's risk
Microbiota and Dysbiosis. Analysis of the microbiome and markers of impaired intestinal permeability.
Inflammatory pathologies. Vitamin D and its metabolome in different pathologies. Vitamin B6 and B12. Methylation and transulfuration cycle: physiological and pathological roles
Next generation sequencing: NGS; study of the whole exome and clinical exome: clinical applications. Proteomics
DNA damage and repair: Comet assay. Widely used techniques in clinical biochemistry, biomedicine, forensics and occupational medicine, diagnostics: immunochemical and electrophoretic techniques, PCR, RFLP. cDNA and notes on vectors. Recombinant DNA and applications: Transgenic plants, biotechnological and biosimilar drugs, cloning from adult cells, edible vaccines

Textbook Information

•Ciaccio-Lippi, Biochimica clinica e medicina di laboratorio. EDISES Eds 2017

•Janson-Tischler, Biochimica Clinica- (Ed Italiana di m. Plebani)- Ediz. Piccin 2015

•Antonozzi, Gulletta, Medicina di Laboratorio Logica e patologia Clinica Piccin

•R. Forlani, Analisi di Laboratorio per operatori della salute, con CD ROM- ENEA Eds 2011


•R.R. Holmes, J,Weyers, A.,Jones: METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI,Ed. Zanichelli 2008;
Damiano Galimberti et al. Nutrigenomica ed epigenetica. Dalla Biologia alla clinica. EdRa 2017
Dai J Dale, et al Geni ai Genomi . Edises 2013