FARMACI BIOLOGICI E BIOTECNOLOGICIModule MODULO 1 CHIMICA FARMACEUTICA
Academic Year 2025/2026 - Teacher: AGOSTINO MARRAZZOExpected Learning Outcomes
Acquire basic knowledge of the methods used in the design and production of biotechnological drugs. This course will include the regulation of gene expression through operons, the protein purification process and its importance in biotechnological drug production, and the purification process of a new recombinant protein from a bacterial culture.
Course Structure
The course consists of 21 hours of face-to-face instruction.
If the course is taught in a blended or distance learning format, any necessary changes to the previously stated curriculum may be made in order to comply with the planned program and the syllabus.
Information for students with disabilities and/or learning disabilities (LDs):
To ensure equal opportunities and in compliance with applicable laws, interested students may request a personal interview to plan any compensatory and/or dispensatory measures, based on their learning objectives and specific needs.
You may also contact the CInAP (Center for Active and Participatory Integration - Services for Disabilities and/or LDs) contact teacher in our Department, Professor Santina Chiechio.
Required Prerequisites
Attendance of Lessons
Detailed Course Content
- Course Contents
- Definition of Pharmaceutical Biotechnology: Multidisciplinary Confluences in Biotechnology.
- Development of Biotechnological Processes and Biotechnology Companies.
- Recombinant DNA Technology. Large-Scale Production of Recombinant Proteins: Design and Production of Biotechnological Drugs.
- Manipulation of Gene Expression in Prokaryotes. Production of Recombinant Proteins in Eukaryotic Cells.
- Analytical and Purification Techniques for Biological Macromolecules.
- Oligonucleotides (ONs): The Medicinal Chemistry of ONs, Pharmacokinetics, and Toxicology. Antisense, Aptamers, Decoys, Antigens, Ribozymes, RNA Interference, and MicroRNAs.
Textbook Information
Reference Texts: During the lecture, the most appropriate texts for studying the topic will be indicated.
Teaching Materials Provided by the Instructor: Studium UniCT
M. L. Calabrò, Compendium of Pharmaceutical Biotechnology, EdiSES.
D. J. A. Crommelin, R. D. Sindelar, B. Meibohm. Pharmaceutical Biotechnology: Fundamentals and Applications, Sixth Edition. Springer.E.
Vegeto, A. Maggi, P. Minghetti, BIOTECHNOLOGICAL DRUGS: Pharmacological and Clinical Aspects, Casa Editrice Ambrosiana.
F. Calabrese and G. Racagni, Principles of Medical and Pharmaceutical Biotechnology. Edra Publishing.
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | Definition of pharmaceutical biotechnology and bioprocess development. | Capitoli 1, 2 Compendio di BIOTECNOLOGIE FARMACEUTICHE, EdiSES. Teaching materials provided by the instructor through the STUDIUM platform. |
| 2 | Recombinant DNA technology. Large-scale production of recombinant proteins: design and production of biotechnological drugs. | Capitolo 1 BIOTECNOLOGIE FARMACEUTICHE, Zanichelli. Capitolo 3 Compendio di BIOTECNOLOGIE FARMACEUTICHE, EdiSES. Teaching materials provided by the instructor through the STUDIUM platform. |
| 3 | Manipulation of gene expression in prokaryotes. Production of recombinant proteins in eukaryotic cells. | Capitoli 6, 7 BIOTECNOLOGIA MOLECOLARE, Zanichelli. Teaching materials provided by the instructor through the STUDIUM platform. |
| 4 | Analytical techniques and purification of biological macromolecules. | Capitolo 7. Compendio di BIOTECNOLOGIE FARMACEUTICHE, EdiSES. |
| 5 | Oligonucleotides (ON): the "medicinal chemistry" of ONs, pharmacokinetics and toxicology. Antisense, aptamers, decoys, antigens, ribozymes, RNA-interference, micro-RNA. Bioinformatics. | Capitolo 5 BIOTECNOLOGIA MOLECOLARE, Zanichelli. Capitolo 7-12 BIOTECNOLOGIE FARMACOLOGICHE, MASSON. eaching materials provided by the instructor through the STUDIUM platform. |
| 6 | In order to pass the exam, there are no minimum required topics; the overall knowledge of required topics will be evaluated. | The Professor provides the material necessary for the study of the topics covered in the classroom through the STUDIUM web application. |
Learning Assessment
Learning Assessment Procedures
Oral exam with a grade out of 30.
The assessment may also be conducted online, if circumstances require.
There are no minimum requirements for passing the exam; basic knowledge of the syllabus topics will be assessed.