ANALISI BIOCHIMICO CLINICO-MOLECOLARI
Academic Year 2020/2021 - 2° YearCredit Value: 6
Scientific field: BIO/12 - Clinical biochemistry and molecular biology
Taught classes: 42 hours
Term / Semester: 2°
Learning Objectives
Dublin descriptors
D1. Knowledge and understanding: of the specific language of the discipline; the main metabolic pathways involved in Laboratory Medicine; identification and practical use of Biomarkers in acute and chronic organ diseases.
D2. Ability to apply knowledge and understanding: knowing how to entertain debates and interactive discussions in the classroom; be able to identify research topics through bibliographic research.
D3. Judgment skills: knowing how to develop and manage a critical reflection on the alteration of some metabolic pathways.
D4. Communication skills: knowing how to better manage communication using a specific technical language of the discipline
D5. Learning skills: acquire adequate skills for the analysis and critical evaluation of biomarkers useful for diagnosis and / or prognosis, with a view to patient-centric approach and medicine 4 or 7P. Demonstrate the ability to search for useful information in clinical and research problem solving; application skills of the acquired knowledge
Course Structure
Interactive Lectures with use of ppt slides containing movie clips, links to Web sites and lecture.
Should teaching be carried out in hybrid mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus
The final exam will be oral
Learning assesment may also be carried out electronically, should the conditions require it.
Detailed Course Content
- Analysis laboratory: organization, diagnostic tests and laboratory tests.
- The biological sample: characteristics; collection, treatment and storage. Conventional biological matrices (blood, urine and faeces) and unconventional (reduced invasiveness: saliva, sweat, hair, nails).
- The analytical cycle: quality control, pre-analytical, analytical, post-analytical and reporting variability. Specificity and Diagnostic Sensitivity (ROC Curve). Predictive value. Cut-off, Reference Range and Decision Levels. Basics of statistics.
- POCT (Point-Of-Care Testing): principles and clinical applications.
- Main analytical methods: theoretical principles, state of development, expected effects in clinical diagnostics and applications in research.
- Biomarkers: What they are and their role in clinical diagnostics. Exogenous and endogenous biochemical markers. Measurement of markers of function and injury of the main organs and tissues (Heart, Pancreas, Liver, Kidney, Muscle, Bone). Markers of pathological processes (acute and chronic inflammation), tumor, neurodegenerative, intestinal pathologies and relations with the Microbiota.
- Liquid biopsy: circulating cells, circulating nucleic acids, miRNAs and exosomes.
- In vivo clinical biochemistry: MR spectroscopy, PET / CT and nuclear tracers. Outline of Radiobiology.
- Methods for monitoring: drugs, toxic agents, substances of abuse and doping in athletes.
- Vitamins and Minerals: dosage in research protocols and in the clinical setting. Mineralogram and validity of its use.
- Notes on Omics Techniques: lipidomics, exome (whole and clinical), exposome, proteome, microbiome, metabolomics, salivomics.
Textbook Information
- Ciaccio-Lippi, Biochimica clinica e medicina di laboratorio. EDISES Eds 2017
- Sacchetti, Cavalcanti, Fortunato, pastore, Rossano, Salvatore, Scopacasa. Medicina di Laboratorio & Diagnostica Genetica. Ed. Sorbona
- Balestrieri C- Diagnostica molecolare nella Medicina di Laboratorio - Vol IX Edizioni Piccin
- LaPosata: Medicina di Laboratorio: diagnosi di malattia nel laboratorio clinico. Ed Piccin